MirGeneDB 2.1

MirGeneDB ID

Lgi-Mir-2-o18

Family name MIR-2 (all species)
Seed AUCACAG
Species Owl limpet (Lottia gigantea)
MiRBase ID
Paralogues Lgi-Mir-2-o14  Lgi-Mir-2-o15  Lgi-Mir-2-o16  Lgi-Mir-2-o17  Lgi-Mir-2-o19 
Orthologues
Node of Origin (locus) L. gigantea
Node of Origin (family) Protostomia
Genome context
(Lotgi1)
LOTGIsca_2: 4514608-4514667 [-] Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o18)
Mir-71 LOTGIsca_2: 4515804-4515862 [-] Ensembl
Mir-2-o14 LOTGIsca_2: 4520084-4520140 [+] Ensembl
Precursor
(pre-Mir +30nt flank)
AAGUUGUUUGUAGAAGGUCAGCUACCCUUCUGACUAAGUGGCUGUGAUAAGUUUAAAAUCAUAGUCUUAUCACAGCCUGCUUAGAUCAGCAUAGAUUCUGACGUACCCUGCUAUUUCUAC
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Structure
        10          20        30          40        50        
AAGUUGUUUGUAGAAGG--    CUACCCUU    -      -|           UUUAAA 
                   UCAG        CUGA CUAAGU GGCUGUGAUAAG      A
                   AGUC        GACU GAUUCG CCGACACUAUUC      U
CAUCUUUAUCGUCCCAUGC    UUAGAUAC    A      U^           UGAUAC 
.       110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes are classified as orphans pending further data and analysis.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
 +
To
Star sequence

Lgi-Mir-2-o18_5p* (predicted)

mirBase accessionNone
Sequence
0- UGACUAAGUGGCUGUGAUAAG -21
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Mature sequence

Lgi-Mir-2-o18_3p

mirBase accessionNone
Sequence
37- UAUCACAGCCUGCUUAGAUCAGC -60
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