MirGeneDB 2.1

MirGeneDB ID

Obi-Mir-2-o22e

Family name MIR-2 (all species)
Seed AUCACAG
Species California two-spot octopus (Octopus bimaculoides)
MiRBase ID
Paralogues Obi-Mir-2-o20-v1  Obi-Mir-2-o20-v2  Obi-Mir-2-o21  Obi-Mir-2-o22a  Obi-Mir-2-o22b  Obi-Mir-2-o22c  Obi-Mir-2-o22d  Obi-Mir-2-o23  Obi-Mir-2-o24 
Orthologues Ovu-Mir-2-o22e 
Node of Origin (locus) Octopus
Node of Origin (family) Protostomia
Genome context
(PRJNA270931_OBI)
KQ419829: 50094-50153 [-] Ensembl
Precursor
(pre-Mir +30nt flank)
CUGUGUUUUGAUGUUCCGACUCUAUUGUAACUCAUUAAAGAGCUGGGAUAUGCUGUCUUUCUGACAUAUCACAGCUAGCUUUGAUGAGCUACACUUGGGUUAUUUCACCUCCAAUUUACU
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Structure
        10          20        30        40          50        
CUGUGUUUUGAUGUUCC--     UAU    A          --|    G      CUGUC 
                   GACUC   UGUA CUCAUUAAAG  AGCUG GAUAUG     U
                   UUGGG   ACAU GAGUAGUUUC  UCGAC CUAUAC     U
UCAUUUAACCUCCACUUUA     UUC    C          GA^    A      AGUCU 
.       110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes are classified as orphans pending further data and analysis. There is a second Drosha cut +1 on the 5p arm.
3' NTU No
MotifsCNNC at 3p(+17)
Tissue expression
 +
To
Star sequence

Obi-Mir-2-o22e_5p*

mirBase accessionNone
Sequence
0- CUCAUUAAAGAGCUGGGAUAUG -22
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Mature sequence

Obi-Mir-2-o22e_3p

mirBase accessionNone
Sequence
36- UAUCACAGCUAGCUUUGAUGAGCU -60
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