Mir-2-o60

MirGeneDB ID

Mgi-Mir-2-o60

Family name

MIR-2 (all species)

Seed

AUCACAG

Species

Pacific oyster (Magallana gigas)

MiRBase ID

Paralogues

Mgi-Mir-2-o56 Mgi-Mir-2-o57 Mgi-Mir-2-o58a Mgi-Mir-2-o58b Mgi-Mir-2-o59 Mgi-Mir-2-o61 Mgi-Mir-2-o62 Mgi-Mir-2-P12

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

C. gigas

Node of Origin (family)

Protostomia

Genome context
(CGI_GCA_000297895.1)

scaffold602: 446019-446076 [+] Ensembl

Precursor
(pre-Mir +30nt flank)

AAGUCGUGGUGAUAAAGAGACAAAUGGAAGUUGUCAAAGUGGUGGUGAUGUGUUGUCAUGUUCGUAUCACAGCCAGCUUUGAUGAGCUUGAAUUUUGUCGCUGUCGGCAGGGGGAGGG

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Structure

        10         20         30          40        50       
AAGUCGUGGUGAUAAAGA-      UGG-     -       -|   G        UUGU 
                   GACAAA    AAGUU GUCAAAG UGGU GUGAUGUG    C
                   CUGUUU    UUCGA UAGUUUC ACCG CACUAUGC    A
GGGAGGGGGACGGCUGUCG      UAAG     G       G^   A        UUGU 
      110       100        90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17)

Tissue expression

A-	D-	D-	Eg	F-	Gi	He	Ju	L-	Ma	Ma	Pe	Re	Sp	Um	Ea	Fe	Ha	La	Ma

Star sequence

Mgi-Mir-2-o60_5p*

mirBase accession

None

Sequence

0- UUGUCAAAGUGGUGGUGAUGUG -22

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Mature sequence

Mgi-Mir-2-o60_3p

mirBase accession

None

Sequence

34- UAUCACAGCCAGCUUUGAUGAGCU -58

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