Mir-2-o46-v1

MirGeneDB ID

Obi-Mir-2-o46-v1

Family name

MIR-2 (all species)

Seed

CACAGCC

Species

California two-spot octopus (Octopus bimaculoides)

MiRBase ID

Paralogues

Obi-Mir-2-o45-v1 Obi-Mir-2-o45-v2 Obi-Mir-2-o46-v2 Obi-Mir-2-o47a Obi-Mir-2-o47b Obi-Mir-2-o47c Obi-Mir-2-o47d Obi-Mir-2-o48-v1 Obi-Mir-2-o48-v2 Obi-Mir-2-o49 Obi-Mir-2-P12

Orthologues

Gsp-Mir-2 Ovu-Mir-2-o46-v1 Ovu-Mir-2-o46-v2 Ple-Mir-2

Node of Origin (locus)

Octopus

Node of Origin (family)

Protostomia

Genome context
(PRJNA270931_OBI)

KQ419829: 51011-51070 [-] Ensembl

Precursor
(pre-Mir +30nt flank)

AUUGCCUAUUCUCUCCUAACCUUGUUACAGCUCAUCAAAGGACUGUGAUAUAUAGAUAUUUGAACGUCUCACAGCCAGCUUUGAUGAGCUGGCUGUAGGUUGCUUCCCUGCUCCGCUAGC

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Structure

        10          20        30           40        50        
AUUGCCUAUUCUCUCCU--     UGUUA            ---| A      UAUAUAGAU 
                   AACCU     CAGCUCAUCAAA   GG CUGUGA         A
                   UUGGA     GUCGAGUAGUUU   CC GACACU         U
CGAUCGCCUCGUCCCUUCG     UGUCG            CGA^ -      CUGCAAGUU 
.       110       100        90        80         70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17)

Tissue expression

To

Star sequence

Obi-Mir-2-o46-v1_5p*

mirBase accession

None

Sequence

0- CUCAUCAAAGGACUGUGAUA -20

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Mature sequence

Obi-Mir-2-o46-v1_3p

mirBase accession

None

Sequence

38- UCACAGCCAGCUUUGAUGAGCU -60

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