MirGeneDB 2.1

MirGeneDB ID

Cin-Let-7-P2o1-v1

Family name LET-7 (all species)
Seed UGAGGUA
Species Sea Squirt (Ciona intestinalis)
MiRBase ID cin-let-7a-1
Paralogues Cin-Let-7-P1  Cin-Let-7-P2o1-v2  Cin-Let-7-P2o2  Cin-Let-7-P2o3  Cin-Let-7-P2o4  Cin-Let-7-P2o5 
Orthologues Aae-Let-7  Bge-Let-7  Bpl-Let-7  Cgi-Let-7  Cte-Let-7  Dan-Let-7  Dma-Let-7  Dme-Let-7  Dmo-Let-7  Dpu-Let-7  Dsi-Let-7  Dya-Let-7  Esc-Let-7  Hme-Let-7  Isc-Let-7  Lan-Let-7  Lgi-Let-7  Npo-Let-7  Obi-Let-7  Ovu-Let-7  Pfl-Let-7  Pmi-Let-7  Sko-Let-7  Spu-Let-7  Tca-Let-7  Xbo-Let-7 
Node of Origin (locus) C. intestinalis
Node of Origin (family) Bilateria
Genome context
(ci3)
4: 2157421-2157502 [+] UCSC Ensembl
Clustered miRNAs
(< 50kb from Let-7-P2o1-v1)
Let-7-P2o1-v1 4: 2157421-2157502 [+] UCSC Ensembl
Let-7-P2o1-v2 4: 2157422-2157502 [+] UCSC Ensembl
Let-7-P2o2 4: 2157552-2157624 [+] UCSC Ensembl
Let-7-P2o3 4: 2157948-2158018 [+] UCSC Ensembl
Let-7-P2o4 4: 2158141-2158206 [+] UCSC Ensembl
Let-7-P2o5 4: 2158340-2158414 [+] UCSC Ensembl
Precursor
(pre-Mir +30nt flank)
GACCACCGAAACAACAUGGCCGUGACCACAAUGAGGUAGUAGGUUAUGCAGUUAUAGCCUCCCUUUUAUACUGAGUGGAGCACAGGAGAUACUGUGUAACCUUCUGCCUUUGUGGCUUCGUGUUUCUAUCAUCAGCUCCCAC
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Structure
        10         20         30        40        50        60        70 
GACCACCGAAACAACAU-   -| UGA     AU       U            UAUAGCCUCCCUUUUAUA 
                  GGC CG   CCACA  GAGGUAG AGGUUAUGCAGU                  C
                  UUG GC   GGUGU  UUCCGUC UCCAAUGUGUCA                  U
CACCCUCGACUACUAUCU   U^ UUC     --       U            UAGAGGACACGAGGUGAG 
140       130       120         110       100        90        80
Deep sequencing
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CommentThis paralogue is related to the vertebrate P2s but it is not yet possible to disentangle the phylogenetic history of vertebrate Let-7 P2s and thus the ascidian genes are classified as orphans pending further data. There is also a second Dicer cut -1 on the 3p arm.
3' NTU Yes
MotifsUG at 5p(-14), CNNC at 3p(+17)
Tissue expression
 +
Cr Cr Cr Cr Cr Cr Cr Cr Cr Cr Ga La La
Mature sequence

Cin-Let-7-P2o1-v1_5p

mirBase accessionMIMAT0006086
Sequence
0- AUGAGGUAGUAGGUUAUGCAGU -22
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Star sequence

Cin-Let-7-P2o1-v1_3p*

mirBase accessionMIMAT0015247
Sequence
62- UGUGUAACCUUCUGCCUUUG -82
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