Mir-2-o161b

MirGeneDB ID

Gsa-Mir-2-o161b

Family name

MIR-2 (all species)

Seed

AUCACAG

Species

Russian doll killer (Gyrodactylus salaris)

MiRBase ID

gsa-mir-2a

Paralogues

Gsa-Mir-2-o161a Gsa-Mir-2-o162 Gsa-Mir-2-o163

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

G. salaris

Node of Origin (family)

Protostomia

Genome context
(gyrodactylus_salaris.PRJNA244375.WBPS19.genomic)

scf7180006951238: 8098-8159 [+] UCSC Ensembl

Clustered miRNAs
(< 50kb from Mir-2-o161b)

Mir-71-o7 scf7180006951238: 7887-7948 [+] UCSC Ensembl
Mir-2-o161a scf7180006951238: 8098-8159 [+] UCSC Ensembl
Mir-2-o161b scf7180006951238: 8098-8159 [+] UCSC Ensembl
Mir-2-o162 scf7180006951238: 8224-8285 [+] UCSC Ensembl

Precursor
(pre-Mir +30nt flank)

CAUUGAACUACAGUCCCAUCUAUUAGGACCGUCCGAGUGCGACUGUGAUGUUAUGUCAUGUUUGCAAUAUCACAGUCCUGCUUUGGUGACGGUGCAAUGAUGGUAUACAGCACCGCUGUAUU

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Structure

        10        20          30           40        50         
CAUUGAACUACAGUCCCAU      GG--      C---|    C            AUGUCA 
                   CUAUUA    ACCGUC    GAGUG GACUGUGAUGUU      \
                   GGUAGU    UGGCAG    UUCGU CUGACACUAUAA      U
UUAUGUCGCCACGACAUAU      AACG      UGGU^    C            CGUUUG 
120       110       100        90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17)

Tissue expression

To

Star sequence

Gsa-Mir-2-o161b_5p*

mirBase accession

MIMAT0035266

Sequence

0- GUCCGAGUGCGACUGUGAUGUU -22

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Mature sequence

Gsa-Mir-2-o161b_3p

mirBase accession

MIMAT0035267

Sequence

37- UAUCACAGUCCUGCUUUGGUGACGG -62

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