Mir-2-o162

MirGeneDB ID

Gsa-Mir-2-o162

Family name

MIR-2 (all species)

Seed

CCCUUGC

Species

Russian doll killer (Gyrodactylus salaris)

MiRBase ID

gsa-mir-2d

Paralogues

Gsa-Mir-2-o161a Gsa-Mir-2-o161b Gsa-Mir-2-o163

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

G. salaris

Node of Origin (family)

Protostomia

Genome context
(gyrodactylus_salaris.PRJNA244375.WBPS19.genomic)

scf7180006951238: 8224-8285 [+] UCSC Ensembl

Clustered miRNAs
(< 50kb from Mir-2-o162)

Mir-71-o7 scf7180006951238: 7887-7948 [+] UCSC Ensembl
Mir-2-o161a scf7180006951238: 8098-8159 [+] UCSC Ensembl
Mir-2-o161b scf7180006951238: 8098-8159 [+] UCSC Ensembl
Mir-2-o162 scf7180006951238: 8224-8285 [+] UCSC Ensembl

Precursor
(pre-Mir +30nt flank)

CAGCAUCUUGCUUAACUGGUGUCUUCAUAAACCCUUGCUCGGGUGUGAUGUGUCUAAUGAUUAAUCAUAUCACAGCCGUGCUUUAAGGGCUUUUGAUGCACCAUAUACUUCAUUAACUGCAA

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Structure

        10           20        30           40        50         
CAGCAUCUUGCUUAACU---     CU   U  A     ---| U   G         UCUAAU 
                    GGUGU  UCA AA CCCUU   GC CGG UGUGAUGUG      \
                    CCACG  AGU UU GGGAA   CG GCC ACACUAUAC      G
AACGUCAAUUACUUCAUAUA     U-   U  C     UUU^ U   G         UAAUUA 
120       110       100         90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

UG at 5p(-14), CNNC at 3p(+17)

Tissue expression

To

Mature sequence

Gsa-Mir-2-o162_5p

mirBase accession

MIMAT0035298

Sequence

0- ACCCUUGCUCGGGUGUGAUGUG -22

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Co-mature sequence

Gsa-Mir-2-o162_3p

mirBase accession

MIMAT0035299

Sequence

37- UAUCACAGCCGUGCUUUAAGGGCUU -62

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