MirGeneDB 3.0
MirGeneDB ID

Gsa-Mir-2-o163

Family name MIR-2 (all species)
Seed AUCACAG
Species Russian doll killer (Gyrodactylus salaris)
MiRBase ID gsa-mir-2b
Paralogues Gsa-Mir-2-o161a  Gsa-Mir-2-o161b  Gsa-Mir-2-o162 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) G. salaris
Node of Origin (family) Protostomia
Genome context
(gyrodactylus_salaris.PRJNA244375.WBPS19.genomic)		 scf7180006953439: 69381-69446 [-] UCSC Ensembl
Clustered miRNAs
(< 50kb from Mir-2-o163)		 Mir-2-o163 scf7180006953439: 69381-69446 [-] UCSC Ensembl
Mir-71-o8 scf7180006953439: 69489-69543 [-] UCSC Ensembl
Precursor
(pre-Mir +30nt flank)
CUGCGUCAGACUGUUCAAUGAUUGAACCUAAGUUGCAAGCGGUUGUUAUAUAAUUUCAUUUUUAAUUCAAUAUAUCACAGCCCUGCUUGGAACGCAAGUUCUUUCUCAAUCUUCUCGAUCGUCCUC
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Structure
        10          20        30        40          50        60 
CUGCGUCAGACUGUUCAAU--  UU    CUAA   G     --|     U     AUUUCAUU 
                     GA  GAAC    GUU CAAGC  GGUUGU AUAUA        U
                     CU  CUUG    CAA GUUCG  CCGACA UAUAU        U
CUCCUGCUAGCUCUUCUAACU  UU    AACG   G     UC^     C     AACUUAAU 
    120       110       100        90        80        70
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsNo
Tissue expression
-   +
Gs
Star sequence

Gsa-Mir-2-o163_5p*
mirBase accessionMIMAT0035272
Sequence 
0- AGUUGCAAGCGGUUGUUAUAUA -22
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Mature sequence

Gsa-Mir-2-o163_3p
mirBase accessionMIMAT0035273
Sequence 
42- UAUCACAGCCCUGCUUGGAACGCA -66
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