Mir-2-o27

MirGeneDB ID

Pdu-Mir-2-o27

Family name

MIR-2 (all species)

Seed

AUCACAG

Species

Dumerils clam worm (Platynereis dumerilii)

MiRBase ID

Paralogues

Pdu-Mir-2-o25-v1 Pdu-Mir-2-o25-v2 Pdu-Mir-2-o26 Pdu-Mir-2-o28 Pdu-Mir-2-o29 Pdu-Mir-2-o30 Pdu-Mir-2-o31 Pdu-Mir-2-o32 Pdu-Mir-2-o33 Pdu-Mir-2-o34 Pdu-Mir-2-o35 Pdu-Mir-2-P12

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

P. dumerilii

Node of Origin (family)

Protostomia

Genome context
(GCA_026936325.1_EMBL_pdum_1.0)

JAPTHN010000003.1: 67087901-67087958 [+] Ensembl

Precursor
(pre-Mir +30nt flank)

UAAAUGAUGCACACAGAACGGUACUCAUGACCAUCUAAGUGGCUGUGAUGUGGUGUUGCGAUGCAUAUCACAGCCAGCUUUGAUGAGCAGGGGUAUUGGCCAAUCAGCUGCUGAUUUC

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Structure

        10          20        30         40        50        
UAAAUGAUGCACACAGAA--        A  AC    U   -|            GUGUU 
                    CGGUACUC UG  CAUC AAG UGGCUGUGAUGUG     \
                    GUUAUGGG AC  GUAG UUC ACCGACACUAUAC     G
CUUUAGUCGUCGACUAACCG        G  GA    U   G^            GUAGC 
      110       100        90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17), UGUG in loop

Tissue expression

24	24	24	24	24	36	36	36	36	36	48	48	48	48	48	6d	6d	6d	6d	72	72	72	72	72

Star sequence

Pdu-Mir-2-o27_5p*

mirBase accession

None

Sequence

0- CCAUCUAAGUGGCUGUGAUGUG -22

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Mature sequence

Pdu-Mir-2-o27_3p

mirBase accession

None

Sequence

35- UAUCACAGCCAGCUUUGAUGAGC -58

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