Mir-2-o33

MirGeneDB ID

Pdu-Mir-2-o33

Family name

MIR-2 (all species)

Seed

AUCACAG

Species

Dumerils clam worm (Platynereis dumerilii)

MiRBase ID

Paralogues

Pdu-Mir-2-o25-v1 Pdu-Mir-2-o25-v2 Pdu-Mir-2-o26 Pdu-Mir-2-o27 Pdu-Mir-2-o28 Pdu-Mir-2-o29 Pdu-Mir-2-o30 Pdu-Mir-2-o31 Pdu-Mir-2-o32 Pdu-Mir-2-o34 Pdu-Mir-2-o35 Pdu-Mir-2-P12

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

P. dumerilii

Node of Origin (family)

Protostomia

Genome context
(GCA_026936325.1_EMBL_pdum_1.0)

JAPTHN010000003.1: 66419072-66419128 [+] Ensembl

Precursor
(pre-Mir +30nt flank)

AAUUAUGAUCGAAUAUUGACCUUUGCAAGGCUGUUGGAUUUGUUGUGACUAUGUAUAAGGCAUCAUAUCACAGCCAAUUUGACAGCCUGGCUUUGGCUCAAAGCGUCACUGAUCUGA

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Structure

        10          20        30        40        50        
AAUUAUGAUCGAAUAUU-  -| UUU  A       UG    U       C    UAUA 
                  GA CC   GC AGGCUGU  GAUU GUUGUGA UAUG    A
                  CU GG   CG UCCGACA  UUAA CGACACU AUAC    G
AGUCUAGUCACUGCGAAA  C^ UUU  G       GU    C       -    UACG 
     110       100        90        80        70         60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

UG at 5p(-14), CNNC at 3p(+17)

Tissue expression

24	24	24	24	24	36	36	36	36	36	48	48	48	48	48	6d	6d	6d	6d	72	72	72	72	72

Star sequence

Pdu-Mir-2-o33_5p*

mirBase accession

None

Sequence

0- CUGUUGGAUUUGUUGUGACUAUG -23

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Mature sequence

Pdu-Mir-2-o33_3p

mirBase accession

None

Sequence

35- UAUCACAGCCAAUUUGACAGCC -57

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