Mir-2-o30

MirGeneDB ID

Pdu-Mir-2-o30

Family name

MIR-2 (all species)

Seed

AUCACAG

Species

Dumerils clam worm (Platynereis dumerilii)

MiRBase ID

Paralogues

Pdu-Mir-2-o25-v1 Pdu-Mir-2-o25-v2 Pdu-Mir-2-o26 Pdu-Mir-2-o27 Pdu-Mir-2-o28 Pdu-Mir-2-o29 Pdu-Mir-2-o31 Pdu-Mir-2-o32 Pdu-Mir-2-o33 Pdu-Mir-2-o34 Pdu-Mir-2-o35 Pdu-Mir-2-P12

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

P. dumerilii

Node of Origin (family)

Protostomia

Genome context
(GCA_026936325.1_EMBL_pdum_1.0)

JAPTHN010000003.1: 67088840-67088898 [+] Ensembl

Precursor
(pre-Mir +30nt flank)

CCUCUCUUUAAGACAUAUGGGUUGGCUAAACUCGCGAAGUCGCUGUGCCAGGUUUUAACGAGUCCUAUCACAGCGUGCUUUGAUGAGCUUAGAUGGCCUGUGAAUCGACCAAGUUCGCU

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Structure

        10          20        30         40         50        
CCUCUCUUUAAGACAUAU--     GG    A    -      -|      CC   UUUUA 
                    GGGUU  CUAA CUCG CGAAGU CGCUGUG  AGG     \
                    UCCGG  GAUU GAGU GUUUCG GCGACAC  UCC     A
UCGCUUGAACCAGCUAAGUG     UA    C    A      U^      UA   UGAGC 
       110       100        90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17)

Tissue expression

24	24	24	24	24	36	36	36	36	36	48	48	48	48	48	6d	6d	6d	6d	72	72	72	72	72

Star sequence

Pdu-Mir-2-o30_5p*

mirBase accession

None

Sequence

0- CUCGCGAAGUCGCUGUGCCAGG -22

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Mature sequence

Pdu-Mir-2-o30_3p

mirBase accession

None

Sequence

35- UAUCACAGCGUGCUUUGAUGAGCU -59

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