Mir-154-P30

MirGeneDB ID

Eca-Mir-154-P30

Family name

MIR-154 (all species)

Seed

GGUUACC

Species

Horse (Equus caballus)

MiRBase ID

eca-mir-409

Paralogues

Eca-Mir-154-P1 Eca-Mir-154-P2-v1 Eca-Mir-154-P2-v2 Eca-Mir-154-P3-v1 Eca-Mir-154-P3-v2 Eca-Mir-154-P4 Eca-Mir-154-P5 Eca-Mir-154-P6a-v1 Eca-Mir-154-P6a-v2 Eca-Mir-154-P6b Eca-Mir-154-P6c Eca-Mir-154-P7 Eca-Mir-154-P8a Eca-Mir-154-P8b Eca-Mir-154-P9 Eca-Mir-154-P10 Eca-Mir-154-P11 Eca-Mir-154-P12 Eca-Mir-154-P16 Eca-Mir-154-P17 Eca-Mir-154-P18 Eca-Mir-154-P19-v1 Eca-Mir-154-P19-v2 Eca-Mir-154-P20 Eca-Mir-154-P21 Eca-Mir-154-P22 Eca-Mir-154-P23 Eca-Mir-154-P24 Eca-Mir-154-P25 Eca-Mir-154-P26 Eca-Mir-154-P27 Eca-Mir-154-P28 Eca-Mir-154-P29 Eca-Mir-154-P31 Eca-Mir-154-P32 Eca-Mir-154-P33 Eca-Mir-154-P34 Eca-Mir-154-P35

Orthologues

Bta-Mir-154-P30 Cfa-Mir-154-P30 Cja-Mir-154-P30 Cpo-Mir-154-P30 Dno-Mir-154-P30 Ete-Mir-154-P30 Hsa-Mir-154-P30 Laf-Mir-154-P30 Mml-Mir-154-P30 Mmr-Mir-154-P30 Mmu-Mir-154-P30 Ocu-Mir-154-P30 Pab-Mir-154-P30 Rno-Mir-154-P30

Node of Origin (locus)

Eutheria

Node of Origin (family)

Eutheria

Genome context
(GCA_002863925.1_EquCab3.0)

CM009171.1: 43374280-43374333 [+] UCSC Ensembl

Clustered miRNAs
(< 50kb from Mir-154-P30)

Mir-154-P1 CM009171.1: 43333278-43333336 [+] UCSC Ensembl
Mir-154-P2-v2 CM009171.1: 43334539-43334596 [+] UCSC Ensembl
Mir-154-P2-v1 CM009171.1: 43334540-43334595 [+] UCSC Ensembl
Mir-154-P3-v1 CM009171.1: 43334998-43335052 [+] UCSC Ensembl
Mir-154-P3-v2 CM009171.1: 43334999-43335051 [+] UCSC Ensembl
Mir-154-P4 CM009171.1: 43336225-43336281 [+] UCSC Ensembl
Mir-154-P5 CM009171.1: 43336787-43336842 [+] UCSC Ensembl
Mir-154-P6a-v1 CM009171.1: 43336949-43337004 [+] UCSC Ensembl
Mir-154-P6a-v2 CM009171.1: 43336949-43337004 [+] UCSC Ensembl
Mir-154-P7 CM009171.1: 43337244-43337301 [+] UCSC Ensembl
Mir-154-P8a CM009171.1: 43338005-43338064 [+] UCSC Ensembl
Mir-154-P8b CM009171.1: 43338319-43338377 [+] UCSC Ensembl
Mir-154-P9 CM009171.1: 43340815-43340870 [+] UCSC Ensembl
Mir-154-P10 CM009171.1: 43341251-43341307 [+] UCSC Ensembl
Mir-154-P11 CM009171.1: 43342885-43342942 [+] UCSC Ensembl
Mir-154-P12 CM009171.1: 43344729-43344786 [+] UCSC Ensembl
Mir-154-P35 CM009171.1: 43346710-43346765 [+] UCSC Ensembl
Mir-376-P2 CM009171.1: 43349741-43349798 [+] UCSC Ensembl
Mir-376-P3 CM009171.1: 43350119-43350176 [+] UCSC Ensembl
Mir-376-P5 CM009171.1: 43350872-43350929 [+] UCSC Ensembl
Mir-154-P16 CM009171.1: 43352997-43353056 [+] UCSC Ensembl
Mir-154-P17 CM009171.1: 43354693-43354755 [+] UCSC Ensembl
Mir-154-P18 CM009171.1: 43355166-43355223 [+] UCSC Ensembl
Mir-154-P19-v1 CM009171.1: 43356005-43356068 [+] UCSC Ensembl
Mir-154-P19-v2 CM009171.1: 43356007-43356066 [+] UCSC Ensembl
Mir-154-P20 CM009171.1: 43356582-43356640 [+] UCSC Ensembl
Mir-154-P21 CM009171.1: 43358248-43358307 [+] UCSC Ensembl
Mir-154-P22 CM009171.1: 43360203-43360256 [+] UCSC Ensembl
Mir-154-P23 CM009171.1: 43360636-43360693 [+] UCSC Ensembl
Mir-154-P24 CM009171.1: 43363504-43363561 [+] UCSC Ensembl
Mir-134 CM009171.1: 43363883-43363944 [+] UCSC Ensembl
Mir-154-P25 CM009171.1: 43364626-43364684 [+] UCSC Ensembl
Mir-154-P26 CM009171.1: 43368798-43368855 [+] UCSC Ensembl
Mir-154-P27 CM009171.1: 43369085-43369145 [+] UCSC Ensembl
Mir-154-P28 CM009171.1: 43369646-43369699 [+] UCSC Ensembl
Mir-154-P29 CM009171.1: 43371377-43371436 [+] UCSC Ensembl
Mir-541 CM009171.1: 43373355-43373420 [+] UCSC Ensembl
Mir-154-P30 CM009171.1: 43374280-43374333 [+] UCSC Ensembl
Mir-154-P31 CM009171.1: 43374430-43374485 [+] UCSC Ensembl
Mir-154-P32 CM009171.1: 43374573-43374628 [+] UCSC Ensembl
Mir-154-P33 CM009171.1: 43374903-43374958 [+] UCSC Ensembl
Mir-154-P34 CM009171.1: 43375650-43375705 [+] UCSC Ensembl

Precursor
(pre-Mir +30nt flank)

CAUCCGAGCCUCUCCAUGGUACUCGGGGAGAGGUUACCCGAGCAACUUUGCAUCUGGACGACGAAUGUUGCUCGGUGAACCCCUUUUCGGUAUCAAAAUCCAUCAGAGGGGCCG

Get precursor sequence

Structure

        10           20        30        40         50      
CAUCCGAGCCUCUCCAU---|    U  GG   A    AC         -    CAUCU 
                    GGUAC CG  GAG GGUU  CCGAGCAAC UUUG     \
                    CUAUG GC  UUC CCAA  GGCUCGUUG AAGC     G
GCCGGGGAGACUACCUAAAA^    -  UU   C    GU         U    AGCAG 
  110       100        90         80        70        60

Deep sequencing

Go to detailed chart

Comment

This gene was classified as a non-canonical miRNA in Fromm et al. (2015) given that the Dicer cut is consistently +4. However, the Drosha cut is +2 and reads are abrogated with the Drosha knock-out and hence it is accepted here.

3' NTU

Motifs

UG at 5p(-14), CNNC at 3p(+17)

Tissue expression

Ps	Bo	Ca	Em	En	En	En	En	En	En	En	Gl	He	He	Ho	Im	Ki	Li	Lo	Lu	Ma	Ma	Mi	Pl	Sp	Te	Tr	Ut	Ut	Yo

Mature sequence

Eca-Mir-154-P30_5p

mirBase accession

MIMAT0013151

Sequence

0- AGGUUACCCGAGCAACUUUGCAU -23

Get sequence

Star sequence

Eca-Mir-154-P30_3p*

mirBase accession

MIMAT0013152

Sequence

32- GAAUGUUGCUCGGUGAACCCCU -54

Get sequence