Mir-154-P30

MirGeneDB ID

Pab-Mir-154-P30

Family name

MIR-154 (all species)

Seed

AAUGUUG

Species

Sumatran orangutan (Pongo abelii)

MiRBase ID

Paralogues

Pab-Mir-154-P1 Pab-Mir-154-P2-v1 Pab-Mir-154-P2-v2 Pab-Mir-154-P3-v1 Pab-Mir-154-P3-v2 Pab-Mir-154-P4 Pab-Mir-154-P5 Pab-Mir-154-P6a-v1 Pab-Mir-154-P6a-v2 Pab-Mir-154-P6b Pab-Mir-154-P7 Pab-Mir-154-P8a Pab-Mir-154-P8b Pab-Mir-154-P9 Pab-Mir-154-P10 Pab-Mir-154-P11 Pab-Mir-154-P12 Pab-Mir-154-P14 Pab-Mir-154-P16a Pab-Mir-154-P16b Pab-Mir-154-P17 Pab-Mir-154-P18 Pab-Mir-154-P19 Pab-Mir-154-P20 Pab-Mir-154-P21 Pab-Mir-154-P23 Pab-Mir-154-P24 Pab-Mir-154-P25 Pab-Mir-154-P26 Pab-Mir-154-P28 Pab-Mir-154-P29 Pab-Mir-154-P31 Pab-Mir-154-P32 Pab-Mir-154-P33 Pab-Mir-154-P34

Orthologues

Bta-Mir-154-P30 Cfa-Mir-154-P30 Cja-Mir-154-P30 Cpo-Mir-154-P30 Dno-Mir-154-P30 Eca-Mir-154-P30 Ete-Mir-154-P30 Hsa-Mir-154-P30 Laf-Mir-154-P30 Mml-Mir-154-P30 Mmr-Mir-154-P30 Mmu-Mir-154-P30 Ocu-Mir-154-P30 Rno-Mir-154-P30

Node of Origin (locus)

Eutheria

Node of Origin (family)

Eutheria

Genome context
(GCA_028885655.2_NHGRI_mPonAbe1-v2.0_traces)

CM054694.2: 105497334-105497387 [+] UCSC Ensembl

Precursor
(pre-Mir +30nt flank)

CAUCUGAGCCUCUCCAUGGUACUCGGGGAGAGGUUACCCGAGCAACUUUGCAUCUGGACGACGAAUGUUGCUCGGUGAACCCCUUUUCGGUAUCAAAUUCCACCAGGGAGGCCG

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Structure

        10           20        30        40         50      
CAUCUGAGCCUCUCCAU---|    U  GG   A    AC         -    CAUCU 
                    GGUAC CG  GAG GGUU  CCGAGCAAC UUUG     \
                    CUAUG GC  UUC CCAA  GGCUCGUUG AAGC     G
GCCGGAGGGACCACCUUAAA^    -  UU   C    GU         U    AGCAG 
  110       100        90         80        70        60

Deep sequencing

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Comment

This gene was classified as a non-canonical miRNA in Fromm et al. (2015) given that the Dicer cut is consistently +4. However, the Drosha cut is +2 and reads are abrogated with the Drosha knock-out and hence it is accepted here.

3' NTU

Motifs

UG at 5p(-14), CNNC at 3p(+17)

Tissue expression

Br	Br	Li	Li

Star sequence

Pab-Mir-154-P30_5p*

mirBase accession

None

Sequence

0- AGGUUACCCGAGCAACUUUGCAU -23

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Mature sequence

Pab-Mir-154-P30_3p

mirBase accession

None

Sequence

32- GAAUGUUGCUCGGUGAACCCCU -54

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