Mir-154-P30

MirGeneDB ID

Rno-Mir-154-P30

Family name

MIR-154 (all species)

Seed

AAUGUUG

Species

Norway rat (Rattus norvegicus)

MiRBase ID

rno-mir-409a

Paralogues

Rno-Mir-154-P1 Rno-Mir-154-P2-v1 Rno-Mir-154-P2-v2 Rno-Mir-154-P3-v1 Rno-Mir-154-P3-v2 Rno-Mir-154-P4 Rno-Mir-154-P6a-v1 Rno-Mir-154-P6a-v2 Rno-Mir-154-P7 Rno-Mir-154-P8-o1 Rno-Mir-154-P9 Rno-Mir-154-P10 Rno-Mir-154-P11 Rno-Mir-154-P12 Rno-Mir-154-P15 Rno-Mir-154-P17 Rno-Mir-154-P18 Rno-Mir-154-P19 Rno-Mir-154-P24 Rno-Mir-154-P25 Rno-Mir-154-P26 Rno-Mir-154-P28-v1 Rno-Mir-154-P28-v2 Rno-Mir-154-P29 Rno-Mir-154-P31 Rno-Mir-154-P32 Rno-Mir-154-P33

Orthologues

Bta-Mir-154-P30 Cfa-Mir-154-P30 Cja-Mir-154-P30 Cpo-Mir-154-P30 Dno-Mir-154-P30 Eca-Mir-154-P30 Ete-Mir-154-P30 Hsa-Mir-154-P30 Laf-Mir-154-P30 Mml-Mir-154-P30 Mmr-Mir-154-P30 Mmu-Mir-154-P30 Ocu-Mir-154-P30 Pab-Mir-154-P30

Node of Origin (locus)

Eutheria

Node of Origin (family)

Eutheria

Genome context
(rn6)

chr6: 133893432-133893485 [+] UCSC Ensembl

Clustered miRNAs
(< 50kb from Mir-154-P30)

Mir-154-P1 chr6: 133857715-133857772 [+] UCSC Ensembl
Mir-154-P2-v2 chr6: 133858858-133858915 [+] UCSC Ensembl
Mir-154-P2-v1 chr6: 133858859-133858914 [+] UCSC Ensembl
Mir-154-P3-v1 chr6: 133859329-133859383 [+] UCSC Ensembl
Mir-154-P3-v2 chr6: 133859330-133859382 [+] UCSC Ensembl
Mir-154-P4 chr6: 133860500-133860556 [+] UCSC Ensembl
Mir-154-P6a-v1 chr6: 133861214-133861269 [+] UCSC Ensembl
Mir-154-P6a-v2 chr6: 133861214-133861269 [+] UCSC Ensembl
Mir-154-P7 chr6: 133861509-133861566 [+] UCSC Ensembl
Mir-154-P8-o1 chr6: 133862190-133862249 [+] UCSC Ensembl
Mir-154-P9 chr6: 133864385-133864440 [+] UCSC Ensembl
Mir-154-P10 chr6: 133864729-133864785 [+] UCSC Ensembl
Mir-666 chr6: 133866541-133866601 [+] UCSC Ensembl
Mir-154-P11 chr6: 133866672-133866729 [+] UCSC Ensembl
Mir-154-P12 chr6: 133868298-133868357 [+] UCSC Ensembl
Mir-667 chr6: 133869584-133869647 [+] UCSC Ensembl
Mir-376-P2 chr6: 133872453-133872510 [+] UCSC Ensembl
Mir-376-P4 chr6: 133873110-133873167 [+] UCSC Ensembl
Mir-376-P5 chr6: 133873618-133873675 [+] UCSC Ensembl
Mir-154-P15 chr6: 133874161-133874223 [+] UCSC Ensembl
Mir-154-P17 chr6: 133876615-133876677 [+] UCSC Ensembl
Mir-154-P18 chr6: 133877137-133877194 [+] UCSC Ensembl
Mir-154-P19 chr6: 133877840-133877901 [+] UCSC Ensembl
Mir-544 chr6: 133879179-133879235 [+] UCSC Ensembl
Mir-154-P24 chr6: 133884190-133884247 [+] UCSC Ensembl
Mir-134 chr6: 133884554-133884614 [+] UCSC Ensembl
Mir-154-P25 chr6: 133885310-133885368 [+] UCSC Ensembl
Mir-154-P26 chr6: 133888600-133888657 [+] UCSC Ensembl
Mir-154-P28-v2 chr6: 133889296-133889351 [+] UCSC Ensembl
Mir-154-P28-v1 chr6: 133889297-133889350 [+] UCSC Ensembl
Mir-154-P29 chr6: 133890697-133890756 [+] UCSC Ensembl
Mir-541 chr6: 133892668-133892733 [+] UCSC Ensembl
Mir-154-P30 chr6: 133893432-133893485 [+] UCSC Ensembl
Mir-154-P31 chr6: 133893579-133893634 [+] UCSC Ensembl
Mir-154-P32 chr6: 133893706-133893761 [+] UCSC Ensembl
Mir-154-P33 chr6: 133894007-133894062 [+] UCSC Ensembl
Mir-3072 chr6: 133898823-133898879 [+] UCSC Ensembl

Precursor
(pre-Mir +30nt flank)

CGUCCAAGCCUCUCCAUGGUACUCGGAGAGAGGUUACCCGAGCAACUUUGCAUCUGGAGGACGAAUGUUGCUCGGUGAACCCCUUUUCGGUAUCAAAUCCCUCCAGGGAGGCCA

Get precursor sequence

Structure

        10           20        30        40         50      
CGUCCAAGCCUCUCCAU---|    U       A    AC         -    CAUCU 
                    GGUAC CGGAGAG GGUU  CCGAGCAAC UUUG     \
                    CUAUG GCUUUUC CCAA  GGCUCGUUG AAGC     G
ACCGGAGGGACCUCCCUAAA^    -       C    GU         U    AGGAG 
  110       100        90         80        70        60

Deep sequencing

Go to detailed chart

Comment

This gene was classified as a non-canonical miRNA in Fromm et al. (2015) given that the Dicer cut is consistently +4. However, the Drosha cut is +2 and reads are abrogated with the Drosha knock-out and hence it is accepted here.

3' NTU

Motifs

UG at 5p(-14), CNNC at 3p(+17)

Tissue expression

Dr	Dr	Ad	Bi	Bi	Bi	Bi	Br	Br	Br	Br	Ce	Ce	Ce	Ce	Co	Co	Co	Co	Du	Du	Du	Du	Fa	He	He	He	Hi	Hi	Il	Il	Je	Je	Ki	Ki	Ki	Ki	Ki	La	Li	Li	Li	Li	Li	Li	Lu	Me	Me	Mu	Ov	Pa	Pa	Pa	Sk	Sm	So	So	So	So	Sp	St	St	St	St	Te	Te	Th	Ut	Wh

Star sequence

Rno-Mir-154-P30_5p*

mirBase accession

MIMAT0003204

Sequence

0- AGGUUACCCGAGCAACUUUGCAU -23

Get sequence

Proposed targets

microrna.org: MIMAT0003204
TargetScanVert: rno-miR-409a-5p
miRDB: MIMAT0003204

Mature sequence

Rno-Mir-154-P30_3p

mirBase accession

MIMAT0003205

Sequence

32- GAAUGUUGCUCGGUGAACCCCU -54

Get sequence

Proposed targets

microrna.org: MIMAT0003205
TargetScanVert: rno-miR-409a-3p
miRDB: MIMAT0003205