Mir-2-o47a-v1

MirGeneDB ID

Esc-Mir-2-o47a-v1

Family name

MIR-2 (all species)

Seed

CACAGCU

Species

Hawaiian bobtail squid (Euprymna scolopes)

MiRBase ID

Paralogues

Esc-Mir-2-o45-v1 Esc-Mir-2-o45-v2 Esc-Mir-2-o47a-v2 Esc-Mir-2-o47b Esc-Mir-2-o47c Esc-Mir-2-o47d Esc-Mir-2-o48-v1 Esc-Mir-2-o48-v2 Esc-Mir-2-o49 Esc-Mir-2-P12

Orthologues

Gsp-Mir-2 Npo-Mir-2-o47 Obi-Mir-2-o47a Ovu-Mir-2-o47a Ple-Mir-2

Node of Origin (locus)

Coleoidea

Node of Origin (family)

Protostomia

Genome context
(GCA_004765925.1_miRNA_LOCI)

Esc-Mir-2-o22h_pre_SRIE01000454.1:382594-382851: 101-158 [+] Ensembl

Precursor
(pre-Mir +30nt flank)

UUUUACCCAAGAGAGAAGGCACUGUUGCAACUCAUCAAAUAGCCGUGAUAUGCUGACAAUGACUUAUCACAGCUAGCUUUGAUGAGCUGGGACUCUGCUCUUUGCUGCUUCUGUUUUA

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Structure

        10          20        30          40        50       
UUUUACCCAAGAGAGAA--    CU   G  A         --|   C      UGCUGA 
                   GGCA  GUU CA CUCAUCAAA  UAGC GUGAUA      C
                   UCGU  CAG GU GAGUAGUUU  AUCG CACUAU      A
AUUUUGUCUUCGUCGUUUC    CU   G  C         CG^   A      UCAGUA 
      110       100        90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

CNNC at 3p(+17)

Tissue expression

Ce	Li

Star sequence

Esc-Mir-2-o47a-v1_5p*

mirBase accession

None

Sequence

0- CUCAUCAAAUAGCCGUGAUA -20

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Mature sequence

Esc-Mir-2-o47a-v1_3p

mirBase accession

None

Sequence

36- UCACAGCUAGCUUUGAUGAGCU -58

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