MirGeneDB 3.0

MirGeneDB ID

Esc-Mir-2-o47b

Family name MIR-2 (all species)
Seed AUCACAG
Species Hawaiian bobtail squid (Euprymna scolopes)
MiRBase ID
Paralogues Esc-Mir-2-o45-v1  Esc-Mir-2-o45-v2  Esc-Mir-2-o47a-v1  Esc-Mir-2-o47a-v2  Esc-Mir-2-o47c  Esc-Mir-2-o47d  Esc-Mir-2-o48-v1  Esc-Mir-2-o48-v2  Esc-Mir-2-o49  Esc-Mir-2-P12 
Orthologues Gsp-Mir-2  Npo-Mir-2-o47  Obi-Mir-2-o47b  Ovu-Mir-2-o47b  Ple-Mir-2 
Node of Origin (locus) Coleoidea
Node of Origin (family) Protostomia
Genome context
(GCA_004765925.1_miRNA_LOCI)
Esc-Mir-2-o22i_pre_SRIE01000454.1:382920-383179: 101-160 [+] Ensembl
Precursor
(pre-Mir +30nt flank)
CACUAUCCUGAGACCAUGACCUUGUUGCAACUCAUCAAAUAGCUGGGAUAUGCUGACAAUAUGACGUAUCACAGCUAGCUUUGAUGAGCUGGGACUAGGUUUCCUCCUCGUCGUUGGUUA
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Structure
        10          20        30          40        50        
CACUAUCCUGAGACCAU--     U   G  A         --|     G      CUGAC 
                   GACCU GUU CA CUCAUCAAA  UAGCUG GAUAUG     A
                   UUGGA CAG GU GAGUAGUUU  AUCGAC CUAUGC     A
AUUGGUUGCUGCUCCUCCU     U   G  C         CG^     A      AGUAU 
.       110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsUG at 5p(-14), CNNC at 3p(+17)
Tissue expression
 +
Li
Star sequence

Esc-Mir-2-o47b_5p*

mirBase accessionNone
Sequence
0- CUCAUCAAAUAGCUGGGAUAUG -22
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Mature sequence

Esc-Mir-2-o47b_3p

mirBase accessionNone
Sequence
36- UAUCACAGCUAGCUUUGAUGAGCU -60
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