Mir-2-o47b

MirGeneDB ID

Esc-Mir-2-o47b

Family name

MIR-2 (all species)

Seed

AUCACAG

Species

Hawaiian bobtail squid (Euprymna scolopes)

MiRBase ID

Paralogues

Esc-Mir-2-o45-v1 Esc-Mir-2-o45-v2 Esc-Mir-2-o47a-v1 Esc-Mir-2-o47a-v2 Esc-Mir-2-o47c Esc-Mir-2-o47d Esc-Mir-2-o48-v1 Esc-Mir-2-o48-v2 Esc-Mir-2-o49 Esc-Mir-2-P12

Orthologues

Gsp-Mir-2 Npo-Mir-2-o47 Obi-Mir-2-o47b Ovu-Mir-2-o47b Ple-Mir-2

Node of Origin (locus)

Coleoidea

Node of Origin (family)

Protostomia

Genome context
(GCA_004765925.1_miRNA_LOCI)

Esc-Mir-2-o22i_pre_SRIE01000454.1:382920-383179: 101-160 [+] Ensembl

Precursor
(pre-Mir +30nt flank)

CACUAUCCUGAGACCAUGACCUUGUUGCAACUCAUCAAAUAGCUGGGAUAUGCUGACAAUAUGACGUAUCACAGCUAGCUUUGAUGAGCUGGGACUAGGUUUCCUCCUCGUCGUUGGUUA

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Structure

        10          20        30          40        50        
CACUAUCCUGAGACCAU--     U   G  A         --|     G      CUGAC 
                   GACCU GUU CA CUCAUCAAA  UAGCUG GAUAUG     A
                   UUGGA CAG GU GAGUAGUUU  AUCGAC CUAUGC     A
AUUGGUUGCUGCUCCUCCU     U   G  C         CG^     A      AGUAU 
.       110       100        90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

UG at 5p(-14), CNNC at 3p(+17)

Tissue expression

Ce	Li

Star sequence

Esc-Mir-2-o47b_5p*

mirBase accession

None

Sequence

0- CUCAUCAAAUAGCUGGGAUAUG -22

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Mature sequence

Esc-Mir-2-o47b_3p

mirBase accession

None

Sequence

36- UAUCACAGCUAGCUUUGAUGAGCU -60

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