Mir-2-o87-v1

MirGeneDB ID

Agr-Mir-2-o87-v1

Family name

MIR-2 (all species)

Seed

CACAGCC

Species

West Indian fuzzy chiton (Acanthopleura granulata )

MiRBase ID

Paralogues

Agr-Mir-2-P12a Agr-Mir-2-P12b Agr-Mir-2-P12c Agr-Mir-2-P12d Agr-Mir-2-P12e Agr-Mir-2-P12f1 Agr-Mir-2-P12f2 Agr-Mir-2-o85 Agr-Mir-2-o86a-v1 Agr-Mir-2-o86a-v2 Agr-Mir-2-o86b Agr-Mir-2-o87-v2

Orthologues

Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

A. granulata

Node of Origin (family)

Protostomia

Genome context
(GCA_016165875.1_ASM1616587v1_Acanthopleura_granula)

JABBOT010000080.1: 1400233-1400291 [+] Ensembl

Precursor
(pre-Mir +30nt flank)

AAUGAUUGUGCAGGACAGGAGGCGACGGUUUCAUCAAAGUGGUGGUGAUGUGUUCUCGUGGUCACAUAUCACAGCCAGCUUUGAUGAGCUCUGUCUACUCCUAUAUCAGUAUCCAGCAU

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Structure

        10         20         30         40        50        
AAUGAUUGUGCAGGACA-    GC     U-          -|   G      UGUUCUC 
                  GGAG  GACGG  UUCAUCAAAG UGGU GUGAUG       G
                  CCUC  CUGUC  GAGUAGUUUC ACCG CACUAU       U
UACGACCUAUGACUAUAU    AU     UC          G^   A      ACACUGG 
       110       100        90        80        70        60

Deep sequencing

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Comment

It is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.

3' NTU

Motifs

UGUG in loop

Tissue expression

To

Star sequence

Agr-Mir-2-o87-v1_5p*

mirBase accession

None

Sequence

0- UCAUCAAAGUGGUGGUGAUG -20

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Mature sequence

Agr-Mir-2-o87-v1_3p

mirBase accession

None

Sequence

38- UCACAGCCAGCUUUGAUGAGC -59

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