MirGeneDB 3.0
MirGeneDB ID

Rph-Mir-2-o66

Family name MIR-2 (all species)
Seed AUCACAG
Species Japanese carpet shell (Ruditapes philippinarum)
MiRBase ID
Paralogues Rph-Mir-2-o63  Rph-Mir-2-o64  Rph-Mir-2-o65  Rph-Mir-2-o67  Rph-Mir-2-o68  Rph-Mir-2-o69  Rph-Mir-2-o70  Rph-Mir-2-o71  Rph-Mir-2-o72  Rph-Mir-2-o73  Rph-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) R. philippinarum
Node of Origin (family) Protostomia
Genome context
(Ruditapes_GCA_009026015)		 CM018536.1: 47737950-47738006 [-] Ensembl
Precursor
(pre-Mir +30nt flank)
UGCAGUGGACAGAAAUUGGGAUAAGUGUUGGCGUCAAAGUGUCUGUGAAAUGUAAAUUAGGUCAUAUCACAGCCAGCUUUGAUGACAGCAAUAUUCUCAGAUCCUUCAUGACAACCU
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Structure
        10          20        30        40         50       
UGCAGUGGACAGAAAUU--    UAAG     G         GU-|     A   UAAA 
                   GGGA    UGUUG CGUCAAAGU   CUGUGA AUG    U
                   CUCU    ACGAC GUAGUUUCG   GACACU UAC    U
UCCAACAGUACUUCCUAGA    UAUA     A         ACC^     A   UGGA 
     110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsUG at 5p(-14), CNNC at 3p(+17)
Tissue expression
-   +
Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru Ru
Star sequence

Rph-Mir-2-o66_5p*
mirBase accessionNone
Sequence 
0- GCGUCAAAGUGUCUGUGAAAUG -22
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Mature sequence

Rph-Mir-2-o66_3p
mirBase accessionNone
Sequence 
34- UAUCACAGCCAGCUUUGAUGACA -57
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