MirGeneDB 3.0
MirGeneDB ID

Rph-Mir-2-o70

Family name MIR-2 (all species)
Seed AUCACAG
Species Japanese carpet shell (Ruditapes philippinarum)
MiRBase ID
Paralogues Rph-Mir-2-o63  Rph-Mir-2-o64  Rph-Mir-2-o65  Rph-Mir-2-o66  Rph-Mir-2-o67  Rph-Mir-2-o68  Rph-Mir-2-o69  Rph-Mir-2-o71  Rph-Mir-2-o72  Rph-Mir-2-o73  Rph-Mir-2-P12 
Orthologues Gsp-Mir-2  Ple-Mir-2 
Node of Origin (locus) R. philippinarum
Node of Origin (family) Protostomia
Genome context
(Ruditapes_GCA_009026015)		 QUSP01002410.1: 60291-60350 [-] Ensembl
Precursor
(pre-Mir +30nt flank)
UGGGAGCAGUUGCAGAGAAAAAUGAAACGCCCGUCAAAGUGGCUGUGAACUGGUGUGUAAUAUUUCAGAUCACAGCCUGCUUUGAUGAGCCUUUCUGAAGCACAUCAGGAUUACACUAUU
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Structure
        10        20          30        40         50         
UGGGAGCAGUUGCAGAGAAAAAU--    C  C         -|       A   GUGUGU 
                         GAAA GC CGUCAAAGU GGCUGUGA CUG      \
                         CUUU CG GUAGUUUCG CCGACACU GAC      A
UUAUCACAUUAGGACUACACGAAGU    C  A         U^       A   UUUAUA 
.       110       100        90        80        70        60
Deep sequencing
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CommentIt is not clear either from phylogenetic or syntenic information how many Mir-2 genes were present in the last common ancestor of protostomes and how the multiple paralogues in lophotrochozoans relate to the four Mir-2 genes in arthropods. Thus all lophotrochozoan genes aside from Mir-2-P12 are classified as orphans pending further data and analysis.
3' NTU No
MotifsUGUG in loop
Tissue expression
-   +
Sr Sr Sr Sr Sr Sr Sr Sr Sr Sr Sr Sr Sr Sr Sr
Star sequence

Rph-Mir-2-o70_5p*
mirBase accessionNone
Sequence 
0- CCGUCAAAGUGGCUGUGAACUG -22
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Mature sequence

Rph-Mir-2-o70_3p
mirBase accessionNone
Sequence 
37- GAUCACAGCCUGCUUUGAUGAGC -60
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