Mir-10-P2b

MirGeneDB ID

Ami-Mir-10-P2b

Family name

MIR-10 (all species)

Seed

ACCCGUA

Species

American alligator (Alligator mississippiensis)

MiRBase ID

ami-mir-99b

Paralogues

Ami-Mir-10-P1b-v1 Ami-Mir-10-P1b-v2 Ami-Mir-10-P1c-v1 Ami-Mir-10-P1c-v2 Ami-Mir-10-P1d-v1 Ami-Mir-10-P1d-v2 Ami-Mir-10-P2c Ami-Mir-10-P2d Ami-Mir-10-P3b Ami-Mir-10-P3c Ami-Mir-10-P3d

Orthologues

Aae-Mir-10-P2 Aca-Mir-10-P2b Bfl-Mir-10-P2-v1 Bfl-Mir-10-P2-v2 Bge-Mir-10-P2 Bla-Mir-10-P2-v1 Bla-Mir-10-P2-v2 Bpl-Mir-10-P2 Bta-Mir-10-P2b Cbr-Mir-10-P2 Cel-Mir-10-P2 Cfa-Mir-10-P2b Cgi-Mir-10-P2 Cin-Mir-10-P2 Cpi-Mir-10-P2b Cpo-Mir-10-P2b Cte-Mir-10-P2 Dan-Mir-10-P2 Dma-Mir-10-P2 Dme-Mir-10-P2 Dmo-Mir-10-P2 Dpu-Mir-10-P2 Dsi-Mir-10-P2 Dya-Mir-10-P2 Ete-Mir-10-P2b Gja-Mir-10-P2b Hsa-Mir-10-P2b Isc-Mir-10-P2 Lan-Mir-10-P2 Lch-Mir-10-P2b Lgi-Mir-10-P2 Mml-Mir-10-P2b Mmu-Mir-10-P2b Mun-Mir-10-P2b Npo-Mir-10-P2 Nve-Mir-10 Oan-Mir-10-P2b Obi-Mir-10-P2 Ocu-Mir-10-P2b Ovu-Mir-10-P2 Pbv-Mir-10-P2b Pfl-Mir-10-P2 Pmi-Mir-10-P2 Rno-Mir-10-P2b Sha-Mir-10-P2b Sko-Mir-10-P2 Spt-Mir-10-P2b Sto-Mir-10-P2b Tca-Mir-10-P2 Xbo-Mir-10-P2 Xbo-Mir-10-P2-as Xla-Mir-10-P2b1 Xla-Mir-10-P2b2 Xtr-Mir-10-P2b

Node of Origin (locus)

Gnathostomata

Node of Origin (family)

Eumetazoa

Genome context
(GCF_000281125.3_ASM28112v4_AMI_add)

NW_017714097.1: 238463-238519 [-] UCSC

Clustered miRNAs
(< 50kb from Mir-10-P2b)

Mir-10-P3b NW_017714097.1: 234135-234193 [-] UCSC
Let-7-P1b NW_017714097.1: 238124-238190 [-] UCSC
Mir-10-P2b NW_017714097.1: 238463-238519 [-] UCSC

Precursor
(pre-Mir +30nt flank)

GCACAACACCCGCGCCUGCCGGUCGCCAUAAACCCGUAGAUCCGAACUUGCGGUCGCUCCUCGUGCAAGCUCGACUCUGUGGGUCUGUGUCGGCCUCGGCCUUGACAUCGGGACGCU

Get precursor sequence

Structure

        10        20          30        40        50        
GCACAACACCCGCGCCUGCC--|    C    A         UC   A      GUCGC 
                      GGUCG CAUA ACCCGUAGA  CGA CUUGCG     \
                      CCGGC GUGU UGGGUGUCU  GCU GAACGU     U
UCGCAGGGCUACAGUUCCGGCU^    U    C         CA   C      GCUCC 
     110       100        90        80        70        60

Deep sequencing

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Comment

Based on the 3' moR reads in human (P2b) and platypus (P2c) it appears that this is a Group 2 miRNA.

3' NTU

Yes

Motifs

UG at 5p(-14)

Tissue expression

To

Mature sequence

Ami-Mir-10-P2b_5p

mirBase accession

MIMAT0038112

Sequence

0- AACCCGUAGAUCCGAACUUGCG -22

Get sequence

Star sequence

Ami-Mir-10-P2b_3p*

mirBase accession

MIMAT0038113

Sequence

35- CAAGCUCGACUCUGUGGGUCUG -57

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