MirGeneDB 3.0
MirGeneDB ID

Eca-Mir-421-P2

Family name MIR-421 (all species)
Seed UCAACAG
Species Horse (Equus caballus)
MiRBase ID eca-mir-421
Paralogues Eca-Mir-421-P1-v1  Eca-Mir-421-P1-v2 
Orthologues Bta-Mir-421-P2  Cfa-Mir-421-P2  Cja-Mir-421-P2  Hsa-Mir-421-P2  Laf-Mir-421-P2  Mml-Mir-421-P2  Mmu-Mir-421-P2  Ocu-Mir-421-P2  Pab-Mir-421-P2  Rno-Mir-421-P2 
Node of Origin (locus) Eutheria
Node of Origin (family) Eutheria
Genome context
(GCA_002863925.1_EquCab3.0)		 CM009179.1: 58556036-58556091 [-] UCSC Ensembl
Clustered miRNAs
(< 50kb from Mir-421-P2)		 Mir-421-P2 CM009179.1: 58556036-58556091 [-] UCSC Ensembl
Mir-374-P2 CM009179.1: 58556200-58556251 [-] UCSC Ensembl
Precursor
(pre-Mir +30nt flank)
ACAGUACCUAAUCCAGUGCACAUUGUAGGCCUCAUUAAAUGUUUGUUGAAUGAAAAAAUGAAUCAUCAACAGACAUUAAUUGGGCGCCUGCUCUGUGAUCUCCAUGAGCUCAGCUU
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Structure
        10         20        30         40        50        
ACAGUACCUAAUCCAGUG-    UU      -|   UUA            A   AAAA 
                   CACA  GUAGGC CUCA   AAUGUUUGUUGA UGA    \
                   GUGU  CGUCCG GGGU   UUACAGACAACU ACU    A
UUCGACUCGAGUACCUCUA    CU      C^   UAA            -   AAGU 
    110       100        90        80        70         60
Deep sequencing
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CommentThere is a second Drosha cut -1 on the 3p arm that is monouridylated at the 3' end.
3' NTU No
MotifsUG at 5p(-14)
Tissue expression
-   +
Er Er Er Er Er Er Er Er Er Er Er Er Er Er Er Er Er Er Er Er
Star sequence

Eca-Mir-421-P2_5p* (predicted)
mirBase accessionMIMAT0013216
Sequence 
0- CUCAUUAAAUGUUUGUUGAAUGA -23
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Mature sequence

Eca-Mir-421-P2_3p
mirBase accessionNone
Sequence 
34- AUCAACAGACAUUAAUUGGGCG -56
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