Let-7-P2b3

MirGeneDB ID

Gja-Let-7-P2b3

Family name

LET-7 (all species)

Seed

GAGGUAG

Species

Schlegels Japanese gecko (Gekko japonicus)

MiRBase ID

Paralogues

Gja-Let-7-P1b Gja-Let-7-P1c Gja-Let-7-P1d Gja-Let-7-P2a1 Gja-Let-7-P2a2 Gja-Let-7-P2a3 Gja-Let-7-P2a4 Gja-Let-7-P2b1 Gja-Let-7-P2b2 Gja-Let-7-P2b4 Gja-Let-7-P2c1 Gja-Let-7-P2c2 Gja-Let-7-P2c3

Orthologues

Aae-Let-7 Aca-Let-7-P2b3 Ami-Let-7-P2b3 Bge-Let-7 Bpl-Let-7 Bta-Let-7-P2b3 Cfa-Let-7-P2b3 Cgi-Let-7 Cpi-Let-7-P2b3 Cpo-Let-7-P2b3 Cte-Let-7 Dan-Let-7 Dma-Let-7 Dme-Let-7 Dmo-Let-7 Dno-Let-7-P2b3 Dpu-Let-7 Dre-Let-7-P2b3a Dsi-Let-7 Dya-Let-7 Esc-Let-7 Ete-Let-7-P2b3 Gmo-Let-7-P2b3a Gmo-Let-7-P2b3b Hme-Let-7 Hsa-Let-7-P2b3 Isc-Let-7 Lan-Let-7 Lgi-Let-7 Loc-Let-7-P2b3 Mal-Let-7-P2b3a Mml-Let-7-P2b3 Mmu-Let-7-P2b3 Mun-Let-7-P2b3 Npo-Let-7 Oan-Let-7-P2b3 Obi-Let-7 Ocu-Let-7-P2b3 Ovu-Let-7 Pbv-Let-7-P2b3 Pfl-Let-7 Pmi-Let-7 Rno-Let-7-P2b3 Sko-Let-7 Spt-Let-7-P2b3 Spu-Let-7 Sto-Let-7-P2b3 Tca-Let-7 Tni-Let-7-P2b3a Tni-Let-7-P2b3b Xbo-Let-7 Xla-Let-7-P2b3c Xla-Let-7-P2b3d Xtr-Let-7-P2b3

Node of Origin (locus)

Gnathostomata

Node of Origin (family)

Bilateria

Genome context
(GCF_001447785.1_Gekko_japonicus)

NW_015173237.1: 128629-128706 [+]

Precursor
(pre-Mir +30nt flank)

GGGGAGGUGUCUCCGUUGCUCCUGCUGAGGUGAGGUAGUAAGUUGUAUUGUUGUGGGGUAGGGAUUUGUGCCCCAAAUCAGAGAUAACUAUACAACUUACUACUUUCCCCAGCUGGGGUGAAGUGCCCAGGAUUUGGG

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Structure

        10          20        30        40        50        60         
GGGGAGGUGUCUCCGUU--|    U    A  UG                U   GUGGGGUAGGGAUUUG 
                   GCUCC GCUG GG  AGGUAGUAAGUUGUAU GUU                \
                   UGGGG CGAC CC  UUCAUCAUUCAACAUA CAA                U
GGGUUUAGGACCCGUGAAG^    U    -  CU                U   UAGAGACUAAACCCCG 
      130       120       110        100        90        80        70

Deep sequencing

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Comment

There is likely a 1 nt mismatch at position 11 on the 5p arm between the source of the genome (G. japonicus) and the source of the reads (C. variegatus) accounting for the paucity of reads of the mature miRNA.

3' NTU

Yes

Motifs

UG at 5p(-14), UGUG in loop

Tissue expression

To

Mature sequence

Gja-Let-7-P2b3_5p

mirBase accession

None

Sequence

0- UGAGGUAGUAAGUUGUAUUGUU -22

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Star sequence

Gja-Let-7-P2b3_3p*

mirBase accession

None

Sequence

57- CUAUACAACUUACUACUUUCC -78

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