MirGeneDB 3.0
MirGeneDB ID

Aca-Mir-216-P2a

Family name MIR-216 (all species)
Seed AAUCUCU
Species Green anole lizard (Anolis carolinensis)
MiRBase ID aca-mir-216b
Paralogues Aca-Mir-216-P2b 
Orthologues Aae-Mir-216-P2  Aga-Mir-216-P2  Ami-Mir-216-P2a  Asu-Mir-216-P2  Ava-Mir-216-P2  Bfl-Mir-216-P2  Bge-Mir-216-P2  Bko-Mir-216-P2  Bla-Mir-216-P2  Bpl-Mir-216-P2  Bta-Mir-216-P2a  Cbr-Mir-216-P2  Cel-Mir-216-P2  Cfa-Mir-216-P2a  Cja-Mir-216-P2a  Cli-Mir-216-P2a  Cmi-Mir-216-P2a  Cpi-Mir-216-P2a  Cpo-Mir-216-P2a  Csc-Mir-216-P2  Dan-Mir-216-P2  Dlo-Mir-216-P2  Dma-Mir-216-P2  Dme-Mir-216-P2  Dmo-Mir-216-P2  Dno-Mir-216-P2a  Dpu-Mir-216-P2  Dre-Mir-216-P2a  Dsi-Mir-216-P2  Dya-Mir-216-P2  Ebu-Mir-216-P2a2  Eca-Mir-216-P2a  Ete-Mir-216-P2a  Gga-Mir-216-P2a  Gja-Mir-216-P2a  Gmo-Mir-216-P2a  Hme-Mir-216-P2  Hsa-Mir-216-P2a  Isc-Mir-216-P2  Laf-Mir-216-P2a  Lch-Mir-216-P2a  Loc-Mir-216-P2a  Mal-Mir-216-P2a  Mdo-Mir-216-P2a  Mml-Mir-216-P2a  Mmr-Mir-216-P2a  Mmu-Mir-216-P2a  Neu-Mir-216-P2a  Oan-Mir-216-P2a  Ocu-Mir-216-P2a  Pab-Mir-216-P2a  Pbv-Mir-216-P2a  Pca-Mir-216-P2  Pma-Mir-216-P2a1  Pma-Mir-216-P2a2  Rno-Mir-216-P2a  Sha-Mir-216-P2a  Spt-Mir-216-P2a  Sto-Mir-216-P2a  Tca-Mir-216-P2  Tgu-Mir-216-P2a  Tni-Mir-216-P2a  Tur-Mir-216-P2 
Node of Origin (locus) Vertebrata
Node of Origin (family) Nephrozoa
Genome context
(anoCar2)		 1: 256248887-256248949 [-] UCSC Ensembl
Clustered miRNAs
(< 50kb from Mir-216-P2a)		 Mir-217-v2 1: 256233879-256233939 [-] UCSC Ensembl
Mir-217-v1 1: 256233880-256233938 [-] UCSC Ensembl
Mir-216-P2b 1: 256236305-256236366 [-] UCSC Ensembl
Mir-216-P2a 1: 256248887-256248949 [-] UCSC Ensembl
Precursor
(pre-Mir +30nt flank)
UUGUGGCCGAAAUCAAAGUCACAGACUGAGAAAUCUCUGCAGGCAAAUGUGAUGUCUUGACCAGCAAUCUCACAGUUACCAGCAGAGGUUCUGCAAUCUGGACUGCUACGGUUAACUGACAUG
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Structure
        10           20         30        40        50        60 
UUGUGGCCGAAAUCAAA---|  A    C  -    A       A  C  A     UGUCUUGA 
                    GUC CAGA UG AGAA UCUCUGC GG AA UGUGA        \
                    CAG GUCU AC UCUU GGAGACG CC UU ACACU        C
GUACAGUCAAUUGGCAUCGU^  -    A  G    -       A  A  G     CUAACGAC 
 120       110        100        90         80        70
Deep sequencing
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3' NTU No
MotifsUGUG in loop
Tissue expression
-   +
Sk Ta Ta To Wh
Mature sequence

Aca-Mir-216-P2a_5p
mirBase accessionMIMAT0021876
Sequence 
0- AAAUCUCUGCAGGCAAAUGUGA -22
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Star sequence

Aca-Mir-216-P2a_3p*
mirBase accessionMIMAT0021877
Sequence 
41- ACAGUUACCAGCAGAGGUUCUG -63
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