Mir-2-P5b

MirGeneDB ID

Aga-Mir-2-P5b

Family name

MIR-2 (all species)

Seed

AAGGAAC

Species

African malaria mosquito (Anopheles gambiae)

MiRBase ID

aga-mir-2944b

Paralogues

Aga-Mir-2-P1-v1 Aga-Mir-2-P1-v2 Aga-Mir-2-P2a Aga-Mir-2-P2b Aga-Mir-2-P3 Aga-Mir-2-P4-v1 Aga-Mir-2-P4-v2 Aga-Mir-2-P5a1 Aga-Mir-2-P5a2 Aga-Mir-2-P7 Aga-Mir-2-P8

Orthologues

Aae-Mir-2-P5b Cel-Mir-2-o5 Dan-Mir-2-P5 Dlo-Mir-2-P5 Dma-Mir-2-P5 Dme-Mir-2-P5 Dmo-Mir-2-P5 Dpu-Mir-2-P5 Dsi-Mir-2-P5 Dya-Mir-2-P5 Gpa-Mir-2-P5-v1 Gpa-Mir-2-P5-v2 Gsp-Mir-2 Ple-Mir-2

Node of Origin (locus)

Culicidae

Node of Origin (family)

Protostomia

Genome context
(GCF_943734735.2_idAnoGambNW_F1_1_genomic)

NC_064602.1: 42670305-42670366 [-] Ensembl

Clustered miRNAs
(< 50kb from Mir-2-P5b)

Mir-2-P5a1 NC_064602.1: 42670118-42670173 [-] Ensembl
Mir-2-P5b NC_064602.1: 42670305-42670366 [-] Ensembl
Mir-279-P1a NC_064602.1: 42670645-42670715 [-] Ensembl

Precursor
(pre-Mir +30nt flank)

AAGUACUCAAAAUCACAGGAAGUGGCGAUCGAAGGAACUCCCGGUGUGAUAUGUUGGAAAAUCCGUACCAUAUCACAGCGGUAGUUACCUGAUAUUGCUGCUGUUCCUCAUUCAACGCGUGC

Get precursor sequence

Structure

        10        20        30         40        50        60 
AAGUACUCAAAAUCACAGGA  UG     CGA   -|   C   G         UUGGAAA 
                    AG  GCGAU   AGG AACU CCG UGUGAUAUG       \
                    UC  CGUUA   UCC UUGA GGC ACACUAUAC       A
CGUGCGCAACUUACUCCUUG  GU     UAG   A^   U   G         CAUGCCU 
120       110       100        90        80        70

Deep sequencing

Go to detailed chart

Comment

mfold supports a different hairpin structure than MirMachine; for the purposes of annotating here, I have recorded the arms based on the MirMachine hairpin

3' NTU

Motifs

Tissue expression

Ab	Ab	He	He	Mi	Mi	Ov	Ov	Ov

Mature sequence

Aga-Mir-2-P5b_5p

mirBase accession

MIMAT0041507

Sequence

0- GAAGGAACUCCCGGUGUGAUAUG -23

Get sequence

Star sequence

Aga-Mir-2-P5b_3p*

mirBase accession

MIMAT0041508

Sequence

40- UAUCACAGCGGUAGUUACCUGA -62

Get sequence