Mir-2-P2a

MirGeneDB ID

Aga-Mir-2-P2a

Family name

MIR-2 (all species)

Seed

AUCACAG

Species

African malaria mosquito (Anopheles gambiae)

MiRBase ID

aga-mir-2c

Paralogues

Aga-Mir-2-P1-v1 Aga-Mir-2-P1-v2 Aga-Mir-2-P2b Aga-Mir-2-P3 Aga-Mir-2-P4-v1 Aga-Mir-2-P4-v2 Aga-Mir-2-P5a1 Aga-Mir-2-P5a2 Aga-Mir-2-P5b Aga-Mir-2-P7 Aga-Mir-2-P8

Orthologues

Bge-Mir-2-P2a Dan-Mir-2-P2a Dlo-Mir-2-P2a Dma-Mir-2-o2 Dma-Mir-2-P2 Dme-Mir-2-P2a Dmo-Mir-2-P2a Dpu-Mir-2-o2 Dpu-Mir-2-P2 Dsi-Mir-2-P2a Dya-Mir-2-P2a Gpa-Mir-2-P2a Gsp-Mir-2 Hme-Mir-2-P2a Ple-Mir-2 Tca-Mir-2-P2a

Node of Origin (locus)

Neoptera

Node of Origin (family)

Protostomia

Genome context
(GCF_943734735.2_idAnoGambNW_F1_1_genomic)

NC_064601.1: 106557252-106557346 [-] Ensembl

Clustered miRNAs
(< 50kb from Mir-2-P2a)

Mir-2-P4-v1 NC_064601.1: 106556282-106556351 [-] Ensembl
Mir-2-P4-v2 NC_064601.1: 106556284-106556349 [-] Ensembl
Mir-2-P3 NC_064601.1: 106556572-106556654 [-] Ensembl
Mir-2-P2b NC_064601.1: 106556743-106556806 [-] Ensembl
Mir-2-P2a NC_064601.1: 106557252-106557346 [-] Ensembl
Mir-2-P1-v1 NC_064601.1: 106557875-106557936 [-] Ensembl
Mir-2-P1-v2 NC_064601.1: 106557875-106557936 [-] Ensembl

Precursor
(pre-Mir +30nt flank)

UGAUGCGAAAAACCACACACAACGGACUGGGCUCAUCAAUUGGUUGUGGCUAUGUGUUUAUCACCAAAUAAACCGGACUCGUUUUCCGCUAUUCACUAUCAUAUCACAGCCAUUUUGAUGAGCUCGGUCGGGUGCUGAAACACAUCUGGUCUCUC

Get precursor sequence

Structure

        10            20        30        40         50        60        70       
UGAUGCGAAAAACCACA----|  AACG               U-         C   GUGUUUAUCACCAAAUAAACCGG 
                     CAC    GACUGGGCUCAUCAA  UGGUUGUGG UAU                       \
                     GUG    CUGGCUCGAGUAGUU  ACCGACACU AUA                       A
CUCUCUGGUCUACACAAAGUC^  GG--               UU         -   CUAUCACUUAUCGCCUUUUGCUC 
   150       140       130         120       110        100        90        80

Deep sequencing

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Comment

There is a second Drosha cut +1 on the 5p arm.

3' NTU

Motifs

CNNC at 3p(+17), UGUG in loop

Tissue expression

Ab	Ab	He	He	Mi	Mi	Ov	Ov	Ov

Star sequence

Aga-Mir-2-P2a_5p*

mirBase accession

MIMAT0041578

Sequence

0- GCUCAUCAAUUGGUUGUGGCUAU -23

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Mature sequence

Aga-Mir-2-P2a_3p

mirBase accession

MIMAT0041579

Sequence

71- UAUCACAGCCAUUUUGAUGAGCUC -95

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